Human antibody capture kit

Human Antibody Capture Kit is intended for capture of human or humanized IgG antibodies as ligands in biomolecular interaction analyses.

• Binds an epitope in CH2 domain of IgG Fc (all subclasses recognized).
• Little or no cross-reactivity towards other human Ig isotypes.
• No, or very low, cross-reactivity with IgG from other species, except other primates.
• Very stable binding - little or no dissociation of captured Abs.
• Sufficient for 10 immobilizations and 1000 regenerations.

Kit contents

• Anti-Human IgG (Fc) antibody, 0.5 mg/mL in: 0.15 M NaCl, 50 µl
• Immobilization buffer(10 mM sodium acetate pH 5.0), 1 mL
• Regeneration solution (3 M MgCl2), 95 mL

Anti-human antibody description

Anti-Human IgG (Fc) consists of a monoclonal mouse anti-human IgG (Fc) antibody of IgG1 isotype, prepared by affinity chromatography on Protein A. The antibody recognizes an epitope within the CH2 domain in human IgG Fc of all subclasses.

Documents

• Instructions for use (PDF)