10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also called Amplex Red or Ampliflu Red, is a sensitive and stable fluorogenic substrate for horseradish peroxidase (HRP). In the presence of HRP and H2O2, ADHP generates highly fluorescent resorufin in red color that has a maximum absorption of 570 nm and maximum emission of 585 nm. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. It has been widely used to detect peroxidase Activity.
Tribioscience’s Peroxidase Activity Fluorometric / Colorimetric Assay provides a quick, efficient, and sensitive method of peroxidase activity.
Applications
Detection of HRP activity on the liquid system.
Key Features
Sensitive and accurate: 10 times more sensitive than TMB substrate for HRP detection.
Simple: Just add- incubate-read style.
Kit Contents
Component
100x Rxns
200x Rxns
ADHP Solution
60 μL
120 μL
Developer
600 μL
1200 μL
HRP standard (1 U/mL)
50 μL
100 μL
1X Assay buffer
10 mL
20 mL
Storage Conditions: Store the kit at -20℃ protected from light.
Shelf Life: 12 months.
Diagram for HRP Standard Preparation
Fluorometric Example Standard Curve: Colorimetric Example Standard Curve:
