HiTrap Con A 4B is a ready-to-use column prepacked with Con A Sepharose 4B, a resin for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.
Con A Sepharose is concanavalin A coupled to Sepharose 4B.
HiTrap Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTA design.
Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides, and glycolipids.
