HiTrap™ chromatography columns with MabSelect™ VL protein L resin . A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability.
High dynamic binding capacity for efficient capture of target molecules.
Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents.
Provides good resolution for product-related impurities in the capture of bispecific antibodies.
Built for a diversifying pipeline
As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.
Redesigned protein L ligand
We observed a two-fold increase in binding capacity of MabSelect™ VL resin compared to our Capto™ L chromatography resin and HiTrap™ Protein L columns. This higher capacity provides productivity advantages in research and biomanufacturing.
The MabSelect™ VL resin can withstand cleaning with 0.1 M NaOH, thanks to multimerization and modification of one of the domains of the native protein L ligand.
