HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
The industry standard for ion exchange chromatography.
High flow rates and good scale-up potential.
Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
Predictable scale-up
Q, SP, DEAE adn CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
