HiTrap™ Chelating HP immobilized metal affinity chromatography columns

HiTrap Chelating HP columns are prepacked with an uncharged IMAC purification resin for purification of proteins and peptides with affinity for metal ions.

• Ready to charge with the metal ion of choice for optimized selectivity.
• Packed with Chelating Sepharose High Performance IMAC resin.
• Convenient affinity purification of native and recombinant proteins, including proteins with a histidine-tag (his-tag).
• Convenient prepacked columns provide fast, simple, and reproducible his-tag protein purification.
• HiTrap format compatible with a syringe, pump or chromatography system such as ÄKTA system or other FPLC system.

Successful use in published research

HiTrap Chelating High Performance is used in a range of research applications, including:

• Purification of glycoproteins on columns charged with copper or zinc ionsto study their post-translational modifications
• Purification of two proteins to study biosynthesis of an [Fe]-hydrogenase cofactor

Other Cytiva IMAC purification resins

Find the right his-tag protein purification product for your application.

Documents

• Data file (PDF)
• Instructions for use (PDF)