HisTALON™ Gravity Column Purification Kit

This kit provides 5 gravity columns, each containing 1 ml of TALON Resin, an immobilized metal affinity chromatography resin for the purification of recombinant his-tagged proteins. These columns may be used under native or denaturing conditions, in combination with a set of equilibration and elution buffers optimized for purifying his-tagged proteins using HisTALON Gravity Columns.

Features

• Eliminating the renaturation step at the end of the purification, saving time, and preventing significant loss of activity
• Retaining the ability to copurify enzyme subunits, cofactors, and associated proteins

Applications

Purified recombinant his-tagged proteins can be used for: Crystallography
Functional assays
Structural investigations
Other applications

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• HisTALON Gravity Column Purification Kit Components List