His GraviTrap TALON® is a prepacked gravity flow column for convenient manual purification of histidine-tagged proteins.
Convenient histidine-tagged purification with high purity (> 90%) and binding capacity of up to 15 mg/column.
Ready-to-use gravity flow column prepacked with 1 mL TALON® Superflow™ cobalt-IMAC (immobilized metal affinity chromatography) resin offering a different selectivity compared to nickel-charged resins.
One run takes about 30 minutes, and large sample volumes can be applied all at once.
His GraviTrap TALON® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged proteins directly from clarified or unclarified cell lysates. His GraviTrap TALON® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography resin from running dry during purification.
Large sample volumes can be applied in one go, and the histidine-tagged protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 mL. Leakage of cobalt ions from TALON® Superflow™ resin is negligible.
