Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.
- Proven for industrial plasma protein purification of various proteins, including antithrombin III antibodies, and coagulation factors.
- Based on Sepharose Fast Flow resins, which are well established in industrial downstream processing. Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
- Heparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Applications of heparin affinity chromatography
Cytiva's heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified.
Chemically stable heparin purification resin
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads, modified with sodium heparin. Immobilization is performed using chemically stable and oriented coupling, which delivers high binding capacity. The chemical stability of the heparin resin is solely dictated by the ligand.
Streamlined Heparin Sepharose Fast Flow scale-up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools.
Successful use in published research
This heparin resin is used in a variety of research applications, including:
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns. Heparin Sepharose 6 Fast Flow is compatible with gravity columns, Tricorn columns, XK columns and HiScale columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. Heparin Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Prepacked Heparin Sepharose Fast Flow columns – research scale
Heparin Sepharose Fast Flow resin is available in the following prepacked formats:
- HiTrap Heparin Sepharose 6 FF: easy to use with a syringe, a peristaltic pump, or a chromatography system
- HiPrep Heparin FF: for convenient scale-up
Prepacked Heparin Sepharose Fast Flow columns – bioprocess scale
Prepacked ReadyToProcess columns are available on request. They eliminate the need for column packing and cleaning validation.
Other Cytiva products for affinity purification of proteins
Looking for a different purification solution? Browse Cytiva's resins and prepacked columns for affinity chromatography or let us help you find the right affinity product.
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