Guide-it™ Recombinant Cas9 (10 µg/µl)

Guide-it Recombinant Cas9 (10 µg/µl) is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease expressed with a C-terminal nuclear localization signal (NLS), provided in 50% glycerol. The Cas9 protein solution has been verified to be sterile and well-tolerated by mammalian cells when electroporated as a ribonucleoprotein complex (RNP) with a single guide RNA (sgRNA) for knockout experiments, or as an RNP with a donor repair template for knockin experiments.

Features

• The Cas9 protein solution is sterile and well-tolerated by mammalian cells
• Highly efficient editing when combined with our Guide-it Complete sgRNA Screening System
• Minimizes off-target effects—better than plasmid-based delivery systems
• Works well in hard-to-transfect cell lines

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)