The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated during cellular non-homologous end joining (NHEJ) repair. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra Direct PCR Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Sufficient material is provided for 100 amplification and cleavage reactions.
Features
Simple method to identify insertions or deletions in mammalian cells
Faster and more efficient than the surveyor assay; amplify directly from your target cells
Complete kit: Guide-it Resolvase, Terra Direct PCR Polymerase, and all buffers included
Value: 100 reactions per kit; also available in a 25-reaction size for small-scale genome editing projects
Perfect for confirming sequence changes downstream from Cas9/CRISPR, TALENs, or zinc-finger nuclease studies
Applications
Confirmation of mutations caused by genome editing technologies, including CRISPR/Cas9, TALENs, or zinc-finger nucleases Detecting insertions and deletions in mammalian genomic DNA
