Guide-it™ CRISPR Genome-Wide sgRNA Library NGS Analysis Kit

The Guide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit enables preparation of next-generation sequencing (NGS) libraries from cell populations screened using the Guide-it CRISPR Genome-Wide sgRNA Library System (Cat. # 632646), allowing for identification of sgRNA sequences and corresponding gene knockouts that have increased or decreased in frequency under the conditions of the screen. The kit provides all the

Features

• Contains >76,000 sgRNAs based on the Brunello library which maximizes on-target specificity and activity, while minimizing off-target effects (download sgRNA representation data)
• Each gene in the human genome is targeted by four highly active sgRNAs, enabling screens with a single module
• Modified sgRNA scaffold design for improved Cas9-sgRNA interaction and high editing efficiency

Applications

Pooled genome-wide phenotypic screens
Loss-of-function genetic screens

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Guide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit Components List