Guide-it™ CRISPR/Cas9 System (Green)

The Guide-it CRISPR/Cas9 System (Green) is a complete system for cloning and expression of a target single guide RNA (sgRNA) for mammalian gene modification studies using CRISPR/Cas9 technology. The pGuide-it-ZsGreen1 Vector is used to simultaneously express Cas9, a target-specific sgRNA, and the exceptionally bright fluorescent protein ZsGreen1. The vector is prelinearized for simple insertion of a target sequence for sgRNA expression from the human U6 promoter; Cas9 nuclease and ZsGreen1 are coexpressed from a bidirectional CMV promoter. This kit also contains all of the

Features

• A single vector expresses your target-specific sgRNA from the human U6 promoter; Cas9 nuclease and a bright fluorescent marker (either ZsGreen1 or tdTomato) are expressed from a bidirectional CMV promoter
• tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6x and 2.5x brighter than EGFP, respectively; these markers can be used to monitor transfection efficiency and/or to enrich/isolate transfected cells by flow cytometry
• Simple plasmid construction—design oligos specific to your target sequence and clone into the pre-linearized plasmid using the included ligation reagents and high-efficiency competent cells
• Includes sufficient reagents for construction of 10 different target (sgRNA) expression plasmids

Applications

Cloning and expression of a target sgRNA for mammalian genome editing using CRISPR/Cas9 technology

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Guide-it CRISPR/Cas9 System (Green) Components List
• pGuide-it-ZsGreen1 (Linear) Vector Sequence in GenBank Format