Guide-it™ Complete sgRNA Screening System

The Guide-it Complete sgRNA Screening System includes everything needed for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies, including PCR reagents for amplifying your genomic target and recombinant Cas9 for in vitro analysis of the transcribed sgRNA.

Features

• Four kits for simple production, cleanup, and evaluation of the efficacy of sgRNA (single guide RNA) for Cas9-mediated gene editing studies
• In vitro transcribe and purify high yields of sgRNA with the Guide-it sgRNA In Vitro Transcription Kit (Cat. # 632635; includes the Guide-it IVT RNA Clean-Up Kit, Cat. # 632638) for transfection or in vitro screening
• Use the Guide-it IVT RNA Clean-Up Kit (Cat. # 632638) to quickly purify sgRNA after IVT reactions, for direct use in transfection, electroporation, or in vitro cleavage assays
• High-quality recombinant Cas9 protein included in the Guide-it sgRNA Screening Kit (Cat. # 632639)

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Guide-it sgRNA In Vitro Transcription Kit Components List
• Components

Kit Components

the necessary reagents for the transcription and cleanup of your sgRNA sequences