GT-T551 is a culture medium ideally suited for expansion of human T lymphocytes (T cells). It is supplemented with human albumin (pharmaceutical grade), recombinant human insulin, L-glutamine, and streptomycin; no additional proteins or growth factors are added. The medium can support stable growth in the presence or absence of serum. Anti-CD3/IL-2 T-cell activation can also be performed in this medium (Wang et al. 2013; Ai et al. 2014; Dodo et al. 2014). When using this activation method, the additional presence of RetroNectin enhances the proliferation of T cells (Yu et al. 2008; Ishikawa et al. 2014; Hosoi et al. 2014; Sakamoto et al. 2015; Li et al. 2015) and results in an expanded cell population that shows a higher proportion of naïve T cells than anti-CD3 antibody stimulation alone (Hosoi et al. 2014).
Features
Used for extended culture or expansion of T cells.
Supplemented with human serum albumin, human insulin, L-glutamine, and streptomycin.
Endotoxin tested (endotoxin <0.3 EU/ml).
Compatible with CultiLife Culture Bags for large-scale expansion of T cells.
The method of T-cell expansion by RetroNectin stimulation requires a license from Takara Bio Inc. for uses other than research.
Applications
Expansion of T cells Gene transfer by lentiviral or retroviral transduction
