Great EscAPe™ SEAP Chemiluminescence Kit 2.0

The Great EscAPe SEAP Chemiluminescence Kit 2.0 is a complete system for the detection of secreted alkaline phosphatase (SEAP) activity in the growth media from mammalian cells expressing this enzyme. The kit contains reagents necessary for the chemiluminescent detection of SEAP activity using a 96-well plate luminometer. The assay can also be performed in single tubes and measured in a tube luminometer. It allows you to monitor promoter activity over time, because samples of the cell media can be taken repeatedly and tested without lysing cells.

Features

• One step assay, for monitoring promoter activity
• 10-fold higher sensitivity then firefly luciferase
• Adaptable from single-tube to high-throughput assays
• Dual, live-cell assay includes secreted luciferase as well as SEAP: monitor two promoters, or use one reporter as a normalization control

Documents

• Safety Data Sheet (PDF)