β-Glucuronidases is a kind of lysosomal enzyme which belongs to the glycosidase family. It plays a critical role during the process of metabolic and detoxification, especially for the breakdown of complex carbohydrates in the human body. β -Glucuronidase level imbalance is related to many diseases, such as cancer, diabetes, liver and detoxification disorders, inflammation, autoimmune, and neuron degenerative diseases. Therefore, it is important to detect activity of β-Glucuronidase in samples to elucidate the pathology of diseases.
Tribioscience’s β-Glucuronidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring glucuronidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p- nitrophenol derivative (R-pNP) as its substrate. The substrate releases a chromophore pNP under β-Glucuronidase, which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of glucuronidase activity in a variety of samples. The reaction was shown in Fig. 1 as below.
Fig.1
Applications
Determination of β-Glucuronidases activity in biological samples.
Key features
Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30-minute reaction at 37˚C
High throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
