Preparation and Specification
Appearance: Yellow powder
Activity: 248 U/mg material
Contaminant: Catalase, Grade I ≤5.0×10⁻³%, GradeⅡ ≤3.0%
Stabilizers: Potassium gluconate, sodium glutamate
Properties
Source: Aspergillus Brasiliensis
Stability: Stable at -20°C for at least 3 years
Molecular weight: approx. 153,000
Michaelis constants: 3.3×10⁻²M (ß-D-Glucose), 6.1×10⁻²M (2-Deoxyglucose)
Structure: Glycoprotein with 2 moles of FAD
Inhibitors: p-Chloromercuribenzoate, heavy metal ions (Cu⁺⁺, Hg⁺⁺, Ag⁺)
pH: 6.01
Optimum temperature: 40-50℃
Thermal stability: below 50℃ (pH 5.7, 1hr)
Lot number: T77910
Solubility: soluble in distilled water or dilute buffer
Unit definition: That amount of enzyme which causes the oxidation of one micromole of glucose per minute at 25℃ and pH 7.0.
Applications
This enzyme is useful for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (if maltooligosaccharide or modified starch is used as a substrate) in clinical analysis.
Research use only.
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