Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅢ 250U/mg-solid or more
Contaminants: NADH oxidase ≤1.0×10⁻³%, α-Glucosidase ≤1.0×10⁻³% Glucose-6-phosphate dehydrogenase ≤1.0×10⁻³%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 101,000 (Gel filtration)
Isoelectric point: 4.5
Michaelis constants: NAD⁺linked: 1.38×10⁻²M (D-Glucose) 3.09×10⁻⁴M (NAD⁺)
NADP⁺linked: 1.25×10⁻²M (D-Glucose) 4.07×10⁻⁵M (NADP⁺)
Inhibitors: Ag⁺, Hg²⁺, Monoiodoacetate
Optimum pH: 9.0
Optimum temperature: 55℃
pH Stability: pH 6.0-7.5 (20℃, 16hr)
Thermal stability: 45℃ (15min-treatment with 50mM K-phosphate buffer, pH 7.0)
Applications
This enzyme is useful for enzymatic determination of D-Glucose.
Research use only.
Documents
