Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅢ 200U/mg-solid or more
Contaminants: Creatine phosphokinase ≤1×10⁻³%
Phosphoglucomutase ≤1×10⁻³%
6-Phosphogluconate dehydrogenase ≤5×10⁻³%
Phosphoglucose isomerase ≤1×10⁻²%
Glutathione reductase ≤1×10⁻³%
Hexokinase ≤1×10⁻²%
Myokinase ≤1×10⁻²%
NADH oxidase ≤1×10⁻²%
NADPH oxidase ≤1×10⁻²%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 140,000 (by gel filtration)
Michaelis constants: NAD⁺ linked 2.4×10⁻⁴M (NAD⁺), 4.7×10⁻⁴M (G-6-P)
NADP⁺ linked 7.4×10⁻⁶M (NADP⁺), 3.2×10⁻⁴M (G-6-P)
Inhibitors: Metal ions, iodoacetamimide, SDS etc.
Optimum pH: 7.8
Optimum temperature: 50°C-55°C
pH Stability: pH 5.0-11.0 (25°C, 22hr)
Thermal stability: below 50°C (pH 7.8, 30min)
Applications
The enzyme is useful for enzymatic determination of NAD⁺(NADP⁺) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase.
Research use only.
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