Tri-Methyl Histone H3K4 refers to the trimethylation of the lysine 4 residue on histone H3. H3K4 tri-methylation has been viewed as a signature mark of highly transcribed genes, which is placed exclusively in the 5’- region downstream of the promoter. Increased global H3K4 tri-methylation is also found to be involved in some pathological processes such as cancer progression. Therefore, quantitative detection of global tri-methyl histone H3K4 would provide useful information for better understanding epigenetic regulation of gene activation, and for developing HMT-targeted drugs.
The Global Tri-Methyl H3K4 Quantification Kit is designed to measure global histone H3K4 tri-methylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, accurate, and fast. The measurement can be completed in 2.5 hours, with a detection range of 20 ng to 5 µg per well.
Standard Preparation
Kit Content and Storage Conditions
PART
48 ASSAYS
96 ASSAYS
STORAGE OF OPENED/ RECONSTITUTED
Detection Antibody
5 µL of histone H3K4 antibody(1000x).
10 µL of histone H3K4 antibody(1000x).
–20°C
Histone H3K4 Standard
10 µL of histone H3K4 control (100ng/µL).
20 µL of histone H3K4 control (100ng/µL).
–20°C
Stop Solution
3 mL of 2 N sulfuric acid.
6 mL of 2 N sulfuric acid.
Room temperature away from light
Wash Buffer
10 mL of concentrated solution (10x).
20 mL of concentrated solution (10x).
Antibody Buffer
6 ml
12 ml
May be stored for up to 6 months at 2-8 °C.
Color Developer
5 ml
10 ml
8-Well Sample Strips (with Frame) Precoated with H3K4 antibody
6
12
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
