H3K4 methylation refers to the addition of methyl groups to the lysine 4 residue of histone H3, a process that can occur in three states: mono-, di-, and trimethylation (H3K4me1, H3K4me2, and H3K4me3). This modification is associated with transcriptional activation and is typically found at the promoter and enhancer regions of actively transcribed genes. H3K4 methylation is a conserved chromatin modification across various organisms, from yeast to humans. H3K4 methylation plays a critical role in gene expression regulation, chromatin structure, and various biological processes, including development and differentiation.
The Global Histone H3K4 Methylation Assay is designed to measure H3-K4 methylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, accurate, and fast. The measurement can be completed in 5 hours, with a detection range of 2 µg to 5 ng per well.
Kit Content and Storage Conditions
PART
48 ASSAYS
96 ASSAYS
STORAGE OF OPENED/ RECONSTITUTED
Detection Antibody
10 µl of histone H3K4 antibody(1000x).
20 µl of histone H3K4antibody(1000x).
–20°C
Methylated H3-K4 Control
10 µL of histone H3K4 control.
20 µL of histone H3K4 control.
–20°C
Wash Buffer
14 ml of concentrated solution (10x).
28 ml of concentrated solution (10x).
Blocking Buffer
10 ml
20 ml
May be stored for up to 6 months at 2-8 °C.
Capture Antibody
25 µl
50 µl
Developing Solution
5 ml
10 ml
8-Well Sample Strips (with Frame) Precoated with H3K4 antibody
6
12
Antibody Buffer
6 ml
12 ml
Room Temperature
10X Lysis Buffer
5 ml
10 ml
Extraction Buffer
8 ml
16 ml
Histone Buffer
0.5 ml
1 ml
Stop Solution
3 ml
6 ml
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
