Acetylation of histone H3 at lysine 9 (H3K9) is a post-translational modification where an acetyl group is added to the lysine residue at position 9 on the histone H3 protein. This modification is mediated by histone acetyltransferases (HATs), which add acetyl groups, and histone deacetylases (HDACs), which remove them. H3K9 acetylation is associated with gene activation. It is often found at active promoters and enhancers, facilitating a more open chromatin structure that allows transcription factors to access DNA. Altered acetylation levels at H3K9 have been linked to various cancers, affecting tumor progression and metastasis.
The Global Acetyl Histone H3K9 Quantification Kit is designed to measure global histone H3K9 acetylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, user-friendly, accurate, and fast. The measurement can be completed in 2.5 hours, with a detection range of 20 ng to 5 µg per well.
Alternative Name: H3K9ac; Acetyl Histone H3K9; Acetylation of histone H3 at lysine 9
Kit Content and Storage Conditions
PART
48 ASSAYS
96 ASSAYS
STORAGE OF OPENED/ RECONSTITUTED
Detection Antibody
5 µL of histone H3K9 antibody(1000x).
10 µL of histone H3K9 antibody(1000x).
–20°C
Histone H3K9 Standard
10 µL of histone H3K9 control.
20 µL of histone H3K9 control.
–20°C
Stop Solution
3 mL of 2 N sulfuric acid.
6 mL of 2 N sulfuric acid.
Room temperature away from light
Wash Buffer
10 mL of concentrated solution (10x).
20 mL of concentrated solution (10x).
Antibody Buffer
6 ml
12 ml
May be stored for up to 6 months at 2-8 °C.
Color Developer
5 ml
10 ml
8-Well Sample Strips (with Frame) Precoated with H3K9 antibody
6
12
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
