Gesicle Producer 293T Cell Line

The Gesicle Producer 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of protein expression. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen. Gesicles are produced in these cells via co-overexpression of packaging mix

Features

• Broad tropism: Efficient delivery of active Cas9 protein and target-specific sgRNA to a broad range of cell types
• Reduced off-target effects: Delivery of Cas9 protein eliminates genomic integration and reduces off-target effects
• Genome editing only when you want it: Tight control over dose and timing of delivery and editing
• The Gesicle Producer 293T Cell Line is not provided with the system and may be purchased separately (Cat. # 632617)
• Stem cell applications: We have demonstrated high-efficiency editing of endogenous genes in human iPS cells using a combination of CRISPR/Cas9 gesicles and our DEF-CS culture system.

Applications

CRISPR/Cas9-mediated gene editing
Stem cell research

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)