The Gene Synthetic Assembly Kit is an enzymatic one-step and isothermal assembly method for gene assembly based on the Gibson Assembly. It is a simple and robust method for assembling multiple fragments of DNA into larger constructs in one step. It allows one to join multiple linear segments into either one large linear segment or, if the segments contain the appropriate components and overlaps, an intact plasmid.
For best results in assembling segments of DNA, ensure segments contain at least 20bp of homology with the segment they are being joined to (Tm of overlapping region must be ≥48°C). Homology overlaps can vary in length from as few as 15bps to 80bps. The efficacy depends on number of fragments assembled.
Applications:
Gene synthetic assembly
Gene cloning
Key Features:
Highly Powerful: One Reaction can assemble multiple gene synthesis.
Highly Efficient: The reaction can be performed in 1 hour.
Streamlined Protocol: Just Add, Mix, and Incubate.
Kit Contents:
Catalog
Name
Kit Size
Reaction
TBS4032-10
2x Gene Assembly
1×100µL
10 RXNs
TBS4032-50
2x Gene Assembly
5×100µL
50 RXNs
Storage Conditions:
The kit is shipped on ice. Store all components at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receiving.
