Faustovirus Capping Enzyme (S17)

Faustovirus Capping Enzyme (S17) adds 7-methylguanylate cap structures (Cap-0) to the 5’ ends of uncapped RNA. Faustovirus Capping Enzyme (25 U/µl), 10X Capping Buffer 2, S-adenosylmethionine (SAM), and GTP are included.

Features

• Generates 7-methylguanylate cap structures (Cap-0) to the 5′ ends of uncapped RNA
• Can be used together with mRNA Cap 2′-O-Methyltransferase (Cat. # 2470A/B) to generate Cap-1 RNA
• Exhibits enzymatic activity over a broader temperature range compared to Vaccinia Capping Enzyme

Applications

Enzymatic capping of in vitro-transcribed mRNA prior to translation
Labeling 5′ ends of mRNA

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)