The Tribo™ Fast Monkey IFN-γ ELISA kit is a solid phase ELISA designed to measure monkey IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature of this kit is its use of a novel fast approach to replace the traditional methods. The measurement can be finished within 1.5 hours instead of the traditional 4-5 hours. The detection range is between 20 and 15,000 pg/mL. The levels of monkey IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural monkey IFN-γ protein.
Kit Contents & Storage Conditions
Assay DiluentTBS3231D 12mL of buffered protein base with preservatives.
Part
Part#
Description
Storage of Opened/Reconstituted
Monkey IFN-γ Microplate
TBS3231A
96-Well Polystyrene Microplate (12-strips of 8-wells) coated with a polyclonal antibody specific for monkey IFN-γ
Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip seal. May be stored for up to 1 month at 2-8℃
Monkey IFN-γ Standard
TBS3231B
0.1mL of Recombinant monkey IFN-γ protein (145.8 ng/mL).
Aliquot and store at -20℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3231C
2.2mL of HRP-Monkey IFN-γ antibody.
May be stored for up to 3 months at 2-8℃
Wash Buffer Concentrate
TBS3000W
12mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6mL of 2N sulfuric acid.
Plate Sealers
N/A
Adhesive Strips
Store the unopened kit at 2-8℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
