Fast Human IL-18 ELISA kit

The Fast Human IL-18 ELISA is a solid phase ELISA designed to measure human IL-18 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human IL-18 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 protein.

 

Key Features:

• Novel Proprietary ELISA Approach
• Fast: Only 2 hr, not 5-6 hrs.
• Simple: Sample and Detection combined into one step.
• Easy: All procedures are performed at room temperature.
• High Sensitivity: The low detection limit is 3.0pg/mL.

The levels of human IL-18 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 protein.

 

Typical Data

This standard curve (R²= 0.9997) is provided for demonstration only in Fig.3. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human IL-18 is typically 3.2 pg/ml.

The Intra-CV is 1.2%, and the Inter-CV is 10.1%.

Specificity

This assay recognizes natural and recombinant human IL-18.

No-cross reactivity: Human IFN-γ; IL-18 BPa/Fc Chimera; IL-18 Rα/Fc Chimera; IL-18 Rβ/Fc Chimera; Canine IL-18; Feline IL-18; Mouse IL-18; Porcine IL-18; Rat IL-18.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human IL-18 Microplate	TBS3239A	96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-18.	The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-18 Standard	TBS3239B	50 µL of Recombinant human IL-18 protein (100ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3239C	2.2 ml of human IL-18 antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3239D	120 µL of Streptavidin-HRP (100x).
Assay Diluent	TBS3239E	25 mL of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use an expired kit for assay.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Product

1. Human IL-1ß ELISA (TBS3219)
2. Human IL-2 ELISA (TBS3220)
3. Human IL-4 ELISA (TBS3221)
4. Human IL-6 ELISA (TBS3223)
5. Human IL-7 ELISA (TBS3224)

 

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)

References

Ao, Z., Cai, H., Wu, Z., Song, S., Karahan, H., Kim, B., Lu, H. C., Kim, J., Mackie, K., & Guo, F. (2021). Tubular human brain organoids to model microglia-mediated neuroinflammation. Lab on a chip, 21(14), 2751–2762. https://doi.org/10.1039/d1lc00030f

Research use only.