The Fast Human Granzyme A ELISA is a solid phase ELISA designed to measure human Granzyme A levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 31 to 2000pg/mL. The levels of human Granzyme A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human Granzyme A protein.
Typical Data
This standard curve(R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human Granzyme A is typically 5 pg/ml.
The Intra-CV is < 10%, the Inter-CV is < 12%.
Specificity
This assay recognizes natural and recombinant human Granzyme A.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Human Granzyme A Microplate
TBS3207A
96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human Granzyme A.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human Granzyme A Standard
TBS3207B
30 μL of Recombinant human Granzyme A protein (100ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3207C
2.1 mL of human Granzyme A antibody.
May be stored for up to
3 months at 2-8 °C.
Detection B
TBS3207D
12 mL of Streptavidin-HRP
Assay Diluent
TBS3207E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
