Exo2 disrupts the Golgi apparatus and stimulates Golgi-ER fusion in mammalian cells. May be used to completely ablate the Golgi apparatus. Like brefeldin A (BFA), it inhibits anterograde transport and disrupts morphology of the trans-Golgi network (TGN) but unlike BFA, it does not induce tubulation and merging of the TGN and endosomal compartments. It perturbs trafficking of Shiga toxin between endosomes and the trans-Golgi network, in contrast to cholera toxin. Accelerates degradation of perilipin-2 proteins.
Application Procedures
First dissolved in DMSO (up to 25 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20°C for up to 1 month.
