Quantitative determination of alcohol or ethanol finds many applications in clinical studies and research, and winery. Studies have shown that heavy alcohol consumption may lead to various forms of liver disease and to increased mortality rates. Tribio Ethanol Colorimetric Assay Kit provides a rapid, simple, reproducible, and sensitive tool for assay ethanol in plasma, serum, urine, and other bio-samples. The ethanol uses a single wording reagent to combine the alcohol oxidase reaction and color reaction into one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to ethanol concentration in the sample. The fluorometric assay is more sensitive than the colorimetric assay.
Applications
Direct Assays: Ethanol concentration in serum, plasma, urine, and other bio-samples.
Key Features
Flexible: Suitable for colorimetric and fluorometric methods.
Accurate: Use 50 µL samples. Detection ranges 0.2-20 mM in 96-well plate for colorimetric assay, and 0.02-10 mM for fluorometric assay.
Simple and high-throughput: One-step procedure: just load-incubate-read. Kit can be used for a robust method.
Time saving: Less than 30 minutes.
Standard Preparation
Typical Data
The typical data is provided for only demonstration reference, shown in Fig.2 and Fig.3.
