EpiScript RNase H-- Reverse Transcriptase (EpiScript RT), is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.
Applications
First-strand cDNA synthesis for subsequent PCR or real-time PCR.
Benefits
Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity Active at temperatures up to 55 °C Highly efficient at producing full-length cDNAs read more
Key features
Produce full-length cDNA with RNAse H-mutant
Active at elevated temperatures up to 55 °C
Specifications
Storage: Store only at -20 °C in a freezer without a defrost cycle.
Storage Buffer: EpiScript RT is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.
Unit Definition: One unit of EpiScript RT catalyses the incorporation of 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37 °C using saturating amounts of oligo(dT)-primed poly(rA) as template.
Contaminating Activity Assays: EpiScript RT is free of detectable exonuclease, endonuclease, and RNase activities.
