EpiScope MSP Kit contains PCR reagents designed exclusively for methylation-specific PCR (MSP). A specific enzyme combined with an optimized buffer allows MSP analyses using a bisulfite-treated DNA template containing uracil. This kit provides a higher amplification efficiency and greatly improved ability to differentiate methylated/unmethylated DNA compared with traditional PCR reagents. The reaction system has been optimized for real-time monitoring using TB Green, and makes it possible to perform both real-time PCR and endpoint detection under the same reaction conditions. Cat. # R100B contains 2 of Cat. # R100A. Please refer to Cat. # R100A for complete product documentation and resources.
Features
High performance: superior amplification speed and efficiency
Specific: differentiate methylated/unmethylated DNA by analyzing a single CpG site
Streamlined: may be used for both real-time (TB Green) and endpoint PCR using the same PCR conditions
Applications
Methylation-specific PCR for DNA methylation analysis
