Enolase is a key glycolytic enzyme in the cytoplasm of cells. It catalyzes the dehydration of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP), in the catabolic glycolytic pathway. Besides its glycolytic function, enolase plays a variety of roles in pathophysiological settings including oncogenesis, tumor progression, ischemia, and bacterial infection. In addition, neuron-specific enolase is released into the cerebrospinal fluid and the systemic circulation upon traumatic brain injury and ischemic episodes. Thus, the measurement of the enolase activity of enolase is a good predictor for patient outcome post cardiac arrest.
Tribioscience’s Enolase Activity Assay is designed to be a flexible, accurate, simple, and time-saving method in which enolase catalyzes 2-PG into PEP, resulting in an intermediate formation through a coupled enzyme reaction. It is catalyzed and reacts with the probe, to generate a colorimetric (570 nm) or fluorometric (Ex/Em=530/590 nm) intensity proportional to the enolase activity which can be measured.
Applications
Direct Assays: Enolase activity in cells, serum, and other biological samples.
