Endotoxin-Free Utrapure Plasmid Extraction Maxiprep is composed of macroporous silica–based ion-exchange resin with a high density of diethylaminoethyl (DEAE) group, purification is based on the interaction between negatively charged phosphates of nucleic acids and positively charged DEAE groups on the surface of the resin, which could efficiently exclude all impurity during the binding-washing-elution process and yield ultrapure plasmid DNA.
Our powerful endotoxin-removal reagent EF Reagent can effectively remove lipopolysaccharides from bacterial cell lysates by phase partition without chilling step. Simply add the EF reagent into the clear cell lysate, and the lipopolysaccharide contaminants can be removed with the flow-through. The Kit System consistently produces endotoxin level less than 0.1 EU/μg DNA of endotoxin-free plasmid DNA product.
Parameter
Value
DNA Fragment length
1.5-kbp ⁓ 150 kbp
Maximal Capacity
>500µg
Maximal Recovery
99%
Minimal Elution Volume
10 mL
Regular Sample Volume
100 mL
Endotoxin Level
Less Than 0.1EU/ μg
Feature
Ion-exchange based columns for rapid and simple extraction.
Reproducible yield of up to 500 µg of endotoxin-free plasmid DNA.
Effective endotoxin removal reagent.
Endotoxin level less than 0.1 EU/µg DNA in the final DNA product.
Application
Transfection of sensitive cells (mammalian primary/suspension cells).
