A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
For use in western blotting, dot, and slot blotting applications
Sensitivity comparable to chemiluminescence
The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
Eliminates need for darkroom and film
Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.
The following reagents are provided, sufficient for detecting 2500 cm² membrane: ECF Substrate, 60 ml, and dilution buffer.
