E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)
Vendor: Omega Bio-tek
SKU: D6943-00
In stock
- Rapid – Purification of plasmid DNA in less than 30 minutes
- Safe – No Phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for a variety of downstream applications
The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats: Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless, whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.
Specifications
| Downstream Application | Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. |
|---|---|
| Starting material | 1-5 mL LB culture |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1-24 |
| DNA binding technology | Silica Mini Spin Column |
| Lysate clearance method | Centrifugation |
| Processing time | <30 minutes |
| Yield | 15-25 µg for high copy-number; 0.1-5 µg for low copy-number |
| Special note | The alternative for discontinued yeast plasmid kit |
Documents
Specifications
- Binding technology Silica Mini Spin Column
- Downstream application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
- Plasmid type High-copy, low-copy, cosmid DNA
- Processing mode Manual (centrifugation or vacuum)
- Processing time <30 minutes
- Starting material 1-5 mL LB culture
- Sub category Bacterial Plasmid
- Throughput 1-24
- Yield 15-25 µg for high copy-number; 0.1-5 µg for low copy-number
links and documants
- Protocol manual Click here
- Quick guide Click here
- Safety data sheet Click here
- Source url Click here
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E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)
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