E.Z.N.A.® Plasmid DNA Mini Kit I, (Q – spin)
Vendor: Omega Bio-tek
SKU: D6942-00
In stock
- Rapid – Purification of plasmid DNA in less than 30 minutes
- Safe – No Phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for a variety of downstream applications
The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. The E.Z.N.A.® Plasmid DNA Mini Kits combine the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA in less than 30 minutes. HiBind® DNA Mini Columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be processed simultaneously.
The E.Z.N.A.® Plasmid DNA Mini Kit is available with two different types of columns. V-spin columns have an attached cap, while Q-spin columns are capless. The columns are otherwise identical in use and application. Both columns can be used with the vacuum or centrifugation protocol.
Specifications
| Downstream Application | Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. |
|---|---|
| Starting material | 1-5 mL LB culture |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1-24 |
| DNA binding technology | Silica Mini Spin Column |
| Lysate clearance method | Centrifugation |
| Processing time | <30 minutes |
| Yield | 15-25 µg for high copy-number; 0.1-5 µg for low copy-number |
Documents
Specifications
- Binding technology Silica Mini Spin Column
- Downstream application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
- Plasmid type High-copy, low-copy, cosmid DNA
- Processing mode Manual (centrifugation or vacuum)
- Processing time <30 minutes
- Starting material 1-5 mL LB culture
- Sub category Bacterial Plasmid
- Throughput 1-24
- Yield 15-25 µg for high copy-number; 0.1-5 µg for low copy-number
links and documants
- Protocol manual Click here
- Quick guide Click here
- Safety data sheet Click here
- Source url Click here
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E.Z.N.A.® Plasmid DNA Mini Kit I, (Q – spin)
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