E.Z.N.A.® MicroElute® Total RNA Kit

SKU: R6831-00

117.00 NIS

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Isolates RNA from small samples with lower elution volumes using spin columns

E.Z.N.A.^® MicroElute^® Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as laser dissected samples (LDS) or fine-needle aspirates (FNA). Normally, up to 5 x 10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows the processing of single or multiple samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10-15 µL nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, poly A+ purification, nuclease protection and in vitro translation.

Specifications

Downstream Application	PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume	15-20 μL
Starting Material	Cultured eukaryotic cells and soft tissues
Starting Amount	Up to 5 x 105 eukaryotic cells or 5 mg tissue
RNA Yield	Up to 50 µg
Processing Mode	Manual, centrifugation
Throughput	1 – 24
RNA Binding Technology	Silica Micro spin column
Binding Capacity	50 µg

Documents

Safety Data Sheet

Specifications

Binding capacity 50 µg
Binding technology Silica Micro spin column
Downstream application PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution volume 15-20 μL
Processing mode Manual, centrifugation
Starting amount Up to 5 x 105 eukaryotic cells or 5 mg tissue
Starting material Cultured eukaryotic cells and soft tissues
Sub category Cultured Cells and Tissue
Throughput 1 – 24
Yield Up to 50 µg

links and documants

Safety data sheet Click here
Source url Click here