E.Z.N.A.® FFPE DNA Kit
Vendor: Omega Bio-tek
SKU: D3399-00
In stock
- Isolate DNA from FFPE samples using spin columns
- Specialized buffer system – High-yielding DNA reversing formalin crosslinking of nucleic acids
- Two paraffin removal methods
- High-quality DNA suitable for qPCR, NGS, etc.
- Cost-effective – Averaging 30% less than competitor
The E.Z.N.A.® FFPE DNA Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method or efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to the MicroElute® DNA column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High quality purified DNA is then eluted in elution buffer or water and is ready for applications such as PCR and next-generation sequencing.
Specifications
| Starting Amount | 3-8 paraffin sections 5-10 µm thick |
|---|---|
| Starting Material | FFPE tissue |
| Elution Volume | 50-75 µL |
| Technology | MicroElute® LE DNA Column |
| Processing Mode | Manual |
| Throughput | 1-24 |
Documents
Specifications
- Binding technology MicroElute® LE DNA Column
- Elution volume 50-75 µL
- Processing mode Manual
- Starting amount 3-8 paraffin sections 5-10 µm thick
- Starting material FFPE tissue
- Sub category Cultured Cells and Tissue
- Throughput 1-24
links and documants
- Protocol manual Click here
- Quick guide Click here
- Safety data sheet Click here
- Source url Click here
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E.Z.N.A.® FFPE DNA Kit
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