E. coli DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of NAD+ as the cofactor. Unlike T4 DNA Ligase, E. coli DNA Ligase can only ligate DNA fragments with cohesive ends under standard reaction conditions. E. coli DNA Ligase is supplied in 10 mM potassium phosphate (pH 7.5), 50 mM KCl, 1 mM DTT, 1 mM EDTA and 50% glycerol. Cat. # 2160B contains 5 of Cat. # 2160A. Please refer to Cat. # 2160A for complete product documentation and resources.
Features
Gene cloning
Phosphodiester bond formation
Applications
Gene cloning Ligation Phosphodiester bond formation
