E. coli DNA Ligase

E. coli DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of NAD+ as the cofactor. Unlike T4 DNA Ligase, E. coli DNA Ligase can only ligate DNA fragments with cohesive ends under standard reaction conditions. E. coli DNA Ligase is supplied in 10 mM potassium phosphate (pH 7.5), 50 mM KCl, 1 mM DTT, 1 mM EDTA and 50% glycerol.

Features

• Gene cloning
• Phosphodiester bond formation

Applications

Gene cloning
Ligation
Phosphodiester bond formation

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)