E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
E. cloni 5-alpha Chemically Competent Cells >1 × 10 8 cfu/µg DNA Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Table 1. E. cloni Competent Cells for Cloning
E. cloni Cell Lines
Transformation Efficiency (cfu/µg pUC DNA)
Cloning Methylated DNA
BAC, Cosmid Cloning
Blue/White Screening
5-alpha Chemically Competent
>1 × 10 8 cfu/µg DNA
NO
NO
YES No IPTG induction required
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Key features
Direct replacements for standard cloning strains
Optimised genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
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Product Details
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
