Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. By reducing the disulfide bonds in a protein sample, the protein can be more effectively fragmented and analyzed. DTT is a commonly used reagent in buffers because of its ability to reduce oxidation of a protein sample, and thereby, preserve enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution. Disulfide reduction is typically complete in minutes at pH 8. Its usefulness stems from its water solubility, reduced odor, and lower toxicity compared to other thiol compounds (2-mercaptoethanol).
Bhaloo, S. I., & Carter, J. E. (2025). Nuclei Isolation from Human Frozen Endometrium with Sample Multiplexing for 10X Genomics Multiome (ATAC + Gene Expression) Assay. protocols.io. https://dx.doi.org/10.17504/protocols.io.ewov12jq2gr2/v1
Research use only.
Specifications
Storage
Supplied as 1M DTT Solution, 50mL. Store at –20℃
