Takara's Dr. GenTLE (from Yeast) High Recovery system is designed to enable the extraction of highly purified DNA from yeast for use in various reactions. The protocol is suitable for simultaneously processing a large number of samples. The kit is composed of three solutions: Dr. GenTLE Solution I lyses cells immediately after being added to the sample, and a noncharged complex is formed with nucleic acids. The complex is then collected by centrifugation, and the precipitate washed with Dr. GenTLE Solution II. Dr. GenTLE Solution III is added to isolate only DNA, which can be precipitated by the addition of isopropanol. The protocol takes just 40 minutes and yields highly purified DNA.
Features
Dr. GenTLE (from Yeast) High Recovery
Quick and consistent yeast genomic DNA extraction in just 40 minutes
Downstream application-ready nucleic acids
Quick and consistent yeast genomic DNA extraction in just 40 minutes
Applications
Dr. GenTLE (from Yeast) High Recovery Quick and consistent yeast genomic DNA extraction in just 40 minutes Downstream application-ready nucleic acids Dr. GenTLE Precipitation Carrier Precipitation of DNA/RNA from even dilute solutions (5 ng/ml nucleic acid) Consistent, high-throughput nucleic acid p
Storage conditions
Dr. GenTLE (from Yeast) High Recovery: Store all components other than Dr. GenTLE Yeast Solution B at 4°C. Store Dr. GenTLE Yeast Solution B at room temperature as low temperature storage may result in formation of a precipitant. If a precipitate forms in
