Dp44mT, Metal chelator

Product Details

Formal Name: 3-(Dipyridin-2-ylmethylideneamino)-1,1-dimethylthiourea

Alternate Names: di-2-pyridylketone-4,4,-dimethyl-3-thiosemicarbazone

Molecular Formula: C₁₄H₁₅N₅S

Formula Weight: 285.37

CAS Number: 152095-12-0

Purity: >98%

Formulation: powder

Solubility: Soluble in DMSO (up to at least 25 mg/ml)

Storage: -20°C

Stability: ≥ 1 year.

Applications

Metal chelator

Functions

A metal chelator with potent antitumor activity. Displays an average IC50 of 30 nM over 28 cancer cell lines (IC50 range 5 nM to 400 nM). Dp44mT retained its antiproliferative activity in both etoposide-resistant MCF-7/VP clones (MCF-7 breast cancer cells) and vinblastine-resistant KB-VB1 clones (KB3-1 epidermoid carcinoma cells) with an IC50 = 12 nM for both lines. The potency of Dp44mT has been attributed to the high redox activity of the Dp44mT-Fe complex leading to cytotoxic ROS generation. The antitumor activity of Dp44mT may also be mediated by a redox active copper complex that causes cellular glutathione depletion and lysosomal damage. It also inhibits T-cell activation and prevented CD25 up-regulation via a copper-dependent mechanism.

Application Procedures

First dissolved in DMSO (up to at least 25 mg/ml), then diluted to aqueous buffer. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)