DH5α Competent Cell (10×50 uL), >10^9

Tribo™ DH5α Competent Cells are an E. coli strain DH5α that provides high transformation efficiencies. Cells can be used in a wide variety of applications for cloning and subcloning. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening of colonies on plates containing X-gal or Bluo-gal. DH5α competent cells support the replication of M13mp vectors but do not support plaque formation.

 

Applications

• General cloning and subcloning.
• Blue-white selection.
• Plasmid DNA amplification.
• Plasmid isolation.

 

Transformation Efficiency (cfu/Ug)
>1×10^⁹

Blue-White Screening
Yes

Reduces Recombination
Yes

Cloning Methylated DNA
No

Improves Plasmid Quality
Yes

recA Deficient:
Yes

Strain
K12

Genotype

F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk-, mk+) phoAsupE44 thi-1 gyrA96 relA1 λ-

 

Storage Conditions

Store the competent cells at -80°C. Shelf life: 6 months.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)