DH10 Competent Cell (10×50 uL), >10^9

Tribo™ E. coli DH10 Competent Cells is an E. coli strain DH10B that provides high transformation efficiencies and allows stable replication of high-copy number plasmids. The competent cells can be used in a wide variety of applications for cloning and subcloning. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening of colonies on plates containing X-gal.

 

Applications

• General cloning and subcloning.
• Blue-white selection.
• Plasmid DNA amplification.
• Plasmid isolation.
• Long-term storage for plasmid stock.

 

Transformation Efficiency (cfu/Ug)
>1×10^⁹

Blue-White Screening
Yes

Reduces Recombination
Yes

Improves Plasmid Quality
Yes

recA Deficient
Yes

Strain
K12

Genotype

F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) endA1 nupG

 

Storage Conditions

Store the competent cells at -80°C. Shelf life: 6 months.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)