Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅢ 100U/mg-solid or more
Contaminants: Malate dehydrogenase ≤2.0×10⁻³%
Lactate dehydrogenase ≤2.0×10⁻³%
NADH oxidase ≤2.0×10⁻³%
Stabilizers: Sucrose, mannitol, BSA
Properties
Stability: Stable at -20°C for at least Two years
Molecular weight: approx. 130,000 (by gel filtration)
Isoelectric point: 5.6±0.1
Michaelis constants: 4.2×10⁻⁴M (25°C, pH8.3), 7.0×10⁻⁴M(37°C, pH8.3)(D-3-Hydroxybutyrate)
4.9×10⁻⁵M (25°C, pH8.3), 7.2×10⁻⁵M (37°C, pH8.3)(NAD⁺)
8.1×10⁻⁵M (25°C, pH7.1), 2.4×10⁻⁴M (37°C, pH7.1)(Acetoacetate)
8.4×10⁻⁶M (25°C, pH7.1), 1.5×10⁻⁵M (37°C, pH7.1)(NADH)
Inhibitors: PCMB, MIA, IAA, Ag⁺, Hg⁺⁺, SDS, DAC
Optimum pH: 8.3
Optimum temperature: 55°C
pH Stability: pH 5.0-8.5 (25°C, 20hr)
Thermal stability: below 40°C (pH 6.5, 15min)
Applications
This enzyme is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.
Research use only.
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