Cytochrome c oxidase [EC 1.9.3.1.] or Complex IV is the fourth complex of the electron Transport Chain located in the mitochondrial, and the principal terminal oxidase of high-affinity oxygen in the aerobic metabolism of all animals, plants, yeasts, and some bacteria. This enzyme is probably unique in providing energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
The colorimetric assay in this kit is based on the observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions.
Components and Storage
This kit is sufficient for 100 tests. Storage at –20°C
| Part Name |
Part Size |
| Assay Buffer, 5 x |
5 mL |
| Enzyme Dilution Buffer, 2x |
2 mL |
| Cytochrome C Solution |
1 mL |
| Dithiothreitol (DTT), 1M |
30 µL |
|
n-Dodecyl β-D-maltoside Buffer |
2 mL |
| Cytochrome c Oxidase Positive Control |
50 µL |
Storage Conditions
The kit ships on wet ice and storage at –20°C is recommended. When stored unopened, the components in this kit are stable for 24 months. After the initial thawing of the 1 M Dithiothreitol Solution, divide the solution into undiluted working aliquots (still at 1 M concentration) and store at -20°C.
Research use only.
Documents
References
1. Hidayat, M., Khaliq, S., Khurram, A., & Lone, K. P. (2019). Protective effects of melatonin on mitochondrial injury and neonatal neuron apoptosis induced by maternal hypothyroidism. Melatonin Research, 2(4):42-60. DOI:10.32794/mr11250040
2. Lin, Y., Hou, X., Shen, W. J., Hanssen, R., Khor, V. K., Cortez, Y., Roseman, A. N., Azhar, S., & Kraemer, F. B. (2016). SNARE-Mediated Cholesterol Movement to Mitochondria Supports Steroidogenesis in Rodent Cells. Molecular endocrinology (Baltimore, Md.), 30(2), 234–247. https://doi.org/10.1210/me.2015-1281
Research use only.
